Primary Research Area:

Biomedical Engineering including Metabolomics

Research Interests:

Tissue engineering, metabolomics, NMR spectroscopy and imaging.

Education:

PhD in Pharmaceutical Chemistry, University of California at San Francisco
MS in Environmental Toxicology, University of California at Davis
BA in Aquatic Biology, University of California at Santa Barbara

About Dr. Jeffrey M. Macdonald:

Dr. Macdonald received his Ph.D. from the Department of Pharmaceutical Chemistry at the University of California at San Francisco where he studied biophysical applications of nuclear magnetic resonance spectroscopy (NMR) and imaging (MRI). He received an undergraduate degree in Aquatic Biology from University of California at Santa Barbara, and a graduate degree in Environmental Toxicology from University of California at Davis. As a Postgraduate Researcher at the Bodega Marine Laboratory (1988-1990) he started his present day interest in NMR/MRI and tissue engineering by designing NMR-compatible perfusion systems for marine organisms for in vivo NMR experiments. Dr. Macdonald was awarded an Intramural Research Training Award (IRTA) fellowship in the Laboratory of Molecular Biophysics and Structural Biology at the National Institute of Environmental Health Sciences (1995-1997), and a National Research Service Award (NRSA) from the National Institute of Diabetes and Digestive and Kidney Diseases (1997-1999). Dr. Macdonald is the Scientific Director of the UNC Metabolomics Facility at Chapel Hill, NC,
and Co-Scientific Director of the NCSU/UNC/NOAA Marine MRI Facility at Beaufort, NC, which
was funded by the North Carolina Biotechnology Center to purchase a narrow-bore 400 MHz NMR
spectrometer and a 40cm horizontal-bore 4.7T MRI, respectively. Dr. Macdonald’s research includes the non-invasive analysis of biological systems by NMR and MRI and the generation of multinuclear NMR metabolomic data at the cellular and systems level, and development of bioartificial liver and other tissue models.

Recent Publications:

1. Nicholas, P. C., Kim, D., Crews, F. T., and Macdonald, J. M. (2008) ”1H NMR-based Metabolomic Analysis of Liver, Serum and Brain Following Ethanol Administration in Rats.” Chem. Res. Tox. 21(2):408-20. Epub 2007 Dec 21. [ Related link ]
2. Li C, Charlton LM, Lakkavaram A, Seagle C, Wang G, Young GB, Macdonald JM, Pielak GJ. (2008). “Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy.” J. Am. Chem. Soc. ;130(20):6310-1. Epub 2008 Apr 18.
3. Seagle, C., Christie, M., Winnike, McClelland, R., J., Ludlow, J. W., O’Connell, T. M., Gamcsik, M. P., and Macdonald, J. M. (2008) “High Through-put Nuclear magnetic Resonance Metabolomic Fingerprinting for Tissue Engineering.” Tiss. Eng. Part C 14(2): 1-12. Epub 2008 May 1. [ Related link ]
4. rner, W.S., Seagle, C., Galanko, J., Favorov, O., Prestwich, G.D., Macdonald, J.M., and L.M. Reid (2008). “Nuclear Magnetic Resonance Metabolomic Footprinting of Human Stem Cells and Hepatoblast Cultured in Hyaluronan-Matrix Hydrogels.” Stem Cell 2008 Jun;26(6):1547-55. Epub 2008 Mar 6.
5. Seagle, C., Christie, M., Winnike, McClelland, R., J., Ludlow, J. W., O’Connell, T. M., Gamcsik, M. P., and Macdonald, J. M. (2008) “High Through-put Metabolomics Fingerprinting for Tissue Engineering.” Tiss. Eng. (in press)
6. Nicholas, P. C., Kim, D., Crews, F. T., and Macdonald, J. M. (2007) ” H NMR-based Metabolomic Analysis of Liver, Serum and Brain Following Ethanol Administration in Rats.” Chem. Res. Tox. 2007 Dec 21 [Epub ahead of print]. [ Related link ]
7. Nicholas, P. C., Kim, D., Crews, F. T., Macdonald, J. M. (2006) “Proton NMR Spectroscopic Determination of Ethanol-Induced Formation of Ethyl Glucuronide in Liver.” Analytical Biochemistry 358 185-191. [ Related link ]
8. Chandrasekaran, P., Seagle, C., Rice, L., Macdonald, J. M., Gerber, D. A. (2006). “Functional Analysis of Encapsulated Hepatic Progenitor Cells” Tissue Engineering 12(7) 1-8.
9. Thelwall, P. E., Yemin,A., Schehl,T., Simpson, N. E., Kasibhatla, M.S., Rabbani, Z. N., Macdonald, J. M., Blackband, S. J., Gamcsik M. P. (2005) “Noninvasive In vivo Detection of Glutathione Metabolism in Tumors.” Can Res. 65(22) 10149-10153
10. McClelland, R. E., Macdonald, J. M., and Coger, R. N. (2003). "Modeling Oxygen Transport Within Engineered Hepatic Devices." Biotech. Bioeng. 82:12-27
11. Macdonald, J. M., Schmidlin, O., and James, T. L. (2002). "In Vivo Monitoring of Hepatic Glutathione in Anesthetized Rats by 13C NMR." Magn. Reson. Med. 48(3):430-439.
12. Wolfe, S. P., Hsu, E., Reid, L. M., and Macdonald, J. M., (2002). "A Novel Multicoaxial Hollow Fiber Bioreactor for Adherent Cell Types. Part I: Hydrodynamic Studies." Biotech. Bioeng. 77:83-90.
13. Macdonald, J. M., Xu, A. S. L, Hiroshi, K., LeCluyse, E., Hamilton, G., Liu, H., Rong, Y.W., Moss, N., Lodestro, C., Luntz, T., Wolfe, S. P., and Reid, L. M. (2001). "Ex Vivo Maintenance of Cells from the Liver Lineage." In "Methods of Tissue Engineering" Lanza, WL, Langer R, and Vacanti, J, eds, San Diego, CA Academic Press; pp 151-201.
14. Macdonald, J. M., Wolfe, S. P., Chowdhury I, Kubota H, and Reid, LM. (2001). "Effect of flow configurationon membrane fouling in a novel multicoaxial hollow fiber bioartificial liver." Ann. N. Y. Acad. Sci. 944:334-343.
15. Wolfe, S. P., Hsu, E., Reid, L. M., and Macdonald, J. M., (2001). "A Novel Multicoaxial Hollow Fiber Bioreactor for Adherent Cell Types. Part I: Hydrodynamic Studies." Biotech. Bioeng. (Accepted upon revision May 26, 2001).
16. Macdonald, J. M., Xu, A. S. L, Hiroshi, K., LeCluyse, E., Hamilton, G., Liu, H., Rong, Y.W., Moss, N., Lodestro, C., Luntz, T., Wolfe, S. P., and Reid, L. M. (2001). "Protocols for isolation and ex vivo Maintenance of Hepatic Stem Cells and Their Normal and Transformed Derivatives." In "Methods of Tissue Engineering" Lanza, WL, Langer R, and Vacanti, J, eds, San Diego, CA Academic Press; (In Press).
17. Macdonald, J. M., Wolfe, S. P., Roy-Chowdhury, I., Hiroshi, K., and Reid, L. M. (2001). "Effect of Flow Configuration and Membrane Characteristics on Membrane Fouling in a Novel Multi-coaxial Hollow Fiber Bioartificial Liver." Ann. N. Y. Acad. Sci. (In Press).
18. Xu, A. S. L., Luntz, T., Macdonald, J. M., Hiroshi, K., Hsu, E., London, R. E., and Reid, L. M. (2000). "Lineage Biology and Liver", In "Principles of Tissue Engineering" Lanza, W.L., Langer R., and Vacanti, J. (editors), Academic Press, San Diego, CA. pp 559-597.
19. Macdonald, J. M., Haas, A., and London, R. E. (2000). "Novel Mechanism of Surface Catalysis of Protein Adduct Formation: NMR Studies of the Acetylation of Ubiquitin." J. Biol. Chem. 275(41):31908-31913.
20. Xu, A. S. L., Macdonald, J. M., Labotka, R. J., and London, R. E. (1999). "Chemical Modification of Human Hemoglobin by Acetyl Salicylic Acid: Sites of Acetylation Studied by NMR." Biochim. Biophys. Acta 1432:333-349.
21. Macdonald, J. M., Griffin, J., Hiroshi K., Griffith, L., Fair, J., and Reid, L. M. (1999). "Bioartificial Livers" In "Cell Encapsulation Technology and Therapeutics", Kuhtreiber, W., Lanza, R. P., and Chick, W. L., (editors), Birkhauser Boston, Cambridge, MA. pp. 252-286.
22. Macdonald, J. M., LeBlanc, D. A., Haas, A., and London, R. E. (1999). "An NMR Analysis of the Reaction of Ubiquitin with [Acetyl-1-13C]Aspirin." Biochem. Pharmacol. 57:1233-1244.
23. Espanol, M. T., Litt, L., Hasegawa, K., Chang, L.-H., Macdonald, J. M., Gregory, G., James, T. L., and Chan, P. H. (1998). Fructose-1,6-Bisphosphate Preserves Adenosine Triphophate but Not Intracellular pH During Hypoxia in Respiring Neonatal Rat Brain Slices." Anesthesiol. 88(2):461-472.
24. Macdonald, J. M., Grillo, M., Schmidlin, O., Tajiri, D. T., and James, T. L. (1998). "NMR Spectroscopy and MRI Investigation of a Potential Bioartificial Liver." Nmr Biomed. 11:55-66.
25. Kurhanewicz, J., Vigneron, D. B., Nelson, S. J., Hricak, H., Macdonald, J. M., Konety, B., and Narayan, P. (1995). "Citrate as an In Vivo Marker to Discriminate Prostatic Cancer from Benign Prostatic Hyperplasia and Normal Prostatic Peripheral Zone: Detection via Localized 1H NMR Spectroscopy." Urology 45(3):459-466.
26. Dahiya, R., Boyle, B., Yoon, W. H., Kurhanewicz, J., Macdonald, J. M., Yen, T.-S. B. and Narayan, P. (1995). "13-Cis-retinoic Acid-mediated Growth Inhibition of DU 145 Human Prostate Cancer Cells." Biochem. Molec. Biol. Int. 32(1):1-12.
27. Macdonald, J. M., Kurhanewicz, J., Dahiya, R., Espanol, M. T., Chang, L.-H., Goldberg, B., James, T. L., and Narayan, P. (1993). "Effect of Glucose and Confluency on Phosphorus Metabolites of Perfused Human Prostatic Adeno-carcinoma Cells as Determined by 31P MRS." Magn. Reson. Med. 29:244-248.
28. Kurhanewicz, J., Dahiya, R., Macdonald, J. M., Chang, L.-H., James, T. L., and Narayan, P. (1993). "Citrate Alterations in DU 145 Xenografts and Primary Human Prostatic Adenocarcinoma: 1H Magnetic Resonance Spectroscopy and Biochemical Study." Magn. Reson. Med. 29:147-157.
29. Higashi, R. M., Cherr G. N., Shenker J. M., Macdonald J. M., and Crosby D. G. (1992). "A Polar High Molecular Mass Constituent of Bleached Kraft Mill Effluent is Toxic to Marine Organisms." Environ. Sci. and Technol. 26:2413-2420.
30. Kurhanewicz, J., Dahiya, R., Macdonald, J. M., Jajodia, P., Chang, L.-H., James, T. L., and Narayan, P., (1992). "Phosphorus Metabolite Characterization of Human Prostatic Adenocarcinoma in a Nude Mouse Model by 31P Magnetic Resonance Spectroscopy and High Pressure Liquid Chromatography." Nmr Biomed. 5:185-192.
31. Narayan, P., Jajodia, P., Kurhanewicz, J., Thomas, A., Macdonald, J. M., Hubesch, B., Hedgecock, M., Andersen, C. M., James, T. L., Tanagho, E. A., and Weiner, M. (1991). "Characterization of Prostatic Hyperplasia and Normal Prostates using Transrectal 31Phosphorus Magnetic Resonance Spectroscopy: A Preliminary Report." J. Urol. 146:66-74.
32. Fan, T. W.-M., Higashi, R. M., and Macdonald, J. M. (1991). "Emergence and Recovery Response of Phosphate Metabolites and Intracellular pH in Intact Mytilus edulis as Examined In Situ by In Vivo 31P-NMR." Biochim. Biophys. Acta 1092:39-47.
33. Higashi, R. M., Fan, T. W.-M., and Macdonald, J. M. (1989). "Monitoring of Metabolic Responses of Intact Haliotis (Abalones) Under Salinity Stress by 31P Surface Probe Localized NMR." J. Exp. Zoo. 249:350-356.

Date of Last Modification: 4/2/2009

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